Functional properties of glycoside synthesized using polyphenolic extract of Moringa oleifera catalyzed through transglycosylation reaction

Abstract

The present study was designed to investigate gallic acid-4-O-β-glucopyranoside (GAGP) in normal and alloxaninduced diabetic rats by a single dose administration of alloxan (150 mg/kg, i.p.). Blood glucose levels measured were significantly (p < 0.05) increasing in all diabetic rats as compared with normal control rats. Three different dosages of GAGP (100, 200, and 400 mg/kg, body weight) showed significantly decreasing (p < 0.05) blood glucose levels of the diabetic rats (<3%) as compared with untreated diabetic group (>66%). All the hyperglycemic groups which were treated with various dosages of GAGP recorded lower biochemical parameters of blood serum as compared with untreated diabetic group. A significant reduction in catalase activity (<40%–60%) of the diabetic groups was observed as compared with control group, while the diabetic groups showed elevation as compared with untreated diabetic group and catalase activity was quite near to normal rats. Glutathione levels of the diabetic groups were reduced (<2%–3%) but diabetic treated groups with different dosage showed improvement near to normal rats. In diabetic rat tissue, significant elevation (>45%) in lipid peroxidation was observed as compared with normal rats. In contrast, treatment with GAGP restores back near towards normal level (>5%–15%).