| dc.description.abstract | Single cell carotenoids (SCC) as microbial metabolite was produced by a growing culture of
yeasts (Phaffia rhodozyma and Rhodotorula minuta) and molds (Neurospora sitophylla and
Monascus purpureus) in coconut water medium containing sucrose. The highest single cell
carotenoid levels had been exhibited by culture of P. Rhodozyma after incubation for 168 h
was approximately 46.12 µg/g, whereas it was shown by M. purpureus, R. minuta and N.
sitophila were 25.30 µg/g, 14.72 µg/g and 12.50 µg/g, respectively. IC50 of 9.9896 µg/ml
was shown by decreasing in absorption of DPPH for each sample of the tested samples that
was measured at every 5 min for 30 min of observation. Although there was a decrease in
absorbance, however, the antioxidant activity of crude SCC derived from culture of N.
sitophila was 22 times lower compared to the antioxidant activity of vitamin C as a positive
control, however, it was higher than the antioxidant activity of the crude SCC derived from
culture of P. rhodozyma, M. purpureus, and R. minuta. This study was to provide important
basic knowledge on physiological aspects of the biosynthesis of SCC derived from microbial
cultures and to assay their antioxidant activities dealing with their potential properties as
an alternative source for natural antioxidant. | en_US |
