HYPOLIPIDEMIC AND ANTIDIABETIC ACTIVITY OF POLYPHENOL GLYCOSIDE CATALYZED BY TRANSGLYCOSYLATION REACTION OF CYCLODEXTRIN GLUCANOTRANSFERASE DERIVED FROM TRICHODERMA VIRIDAE IN DIABETIC RATS

Abstract

The study aimed to synthesis polyphenol glycoside through enzymatic transglycosylation reaction using cyclodextrin glucanotransferase (CGTase) derived from culture of Trichoderma viride in the present of polyphenolic extract of Moringa oleifera to evaluate its antioxidant activities of the polyphenol glycoside as transglycosylation product. Chemical structures of the enzymatically synthesized product were identified using nuclear magnetic resonance ( 1H NMR and 13C NMR) spectroscopy as gallic acid-4-O-βglucopyranoside (GAGP), ellagic acid-4-O-β-glucopyranoside (EAGP), and catechin-4’-O-glucopyranoside (CGP), respectively. The EAGP was furthermore applied to investigate its effect on hypolipidemic and antidiabetic activity in normal and alloxan-induced diabetic rats. Blood glucose levels were measured significantly (p<0.05) increasing in all diabetic rats as compared to normal control rats. Three different dosages of EAGP showed significantly decreasing (p<0.05) blood glucose levels of the diabetic rats as compared to untreated diabetic group. All diabetic groups presented significantly lower body weight as compared to normal rats as control. The diabetic groups administered with different dosages of EAGP showed improvement in weight gain as compared to untreated diabetic group. All the hyperglycemic groups which were treated with EAGP recorded lower biochemical parameters of blood serum as compared to untreated diabetic group