| dc.description.abstract | Simultaneous purification of fucoxanthin isomers from brown seaweeds by two steps of open-column chromatography (OCC) and reversed-phase (RP)-high-performance liquid chromatography (HPLC) is described. Analysis
and identification of fucoxanthin isomers were performed by chromatographic and spectrophotometric properties such as retention time (tR), spectral shape, maximal absorption wavelength (λmax), Q-ratio, and mass
spectrometry (MS) data including the ratio of fragment ions. The optimal conditions for a simultaneous separation and purification were examined by changing several parameters of HPLC, i.e., mobile phase composition,
equilibration time, and column oven temperature. The purification procedure consisted of the following two
steps: first, highly purified fucoxanthin fraction was obtained by a silica-gel OCC. Then, four major fucoxanthin
isomers, all-trans, 13ʹ-cis, 13-cis, and 9ʹ-cis, were simultaneously separated and purified by RP-HPLC with an
analytical C30 column and gradient elution in a mixture of water, methanol, and methyl tert-butyl ether. The
purity of fucoxanthin isomers purified was >95% for all-trans and 9ʹ-cis, 85% for 13ʹ-cis, and >80% for 13-cis. A
large-scale purification by RP-HPLC using a preparative C18 column was effective for the purification of all-trans
and 9ʹ-cis with a yield of 95%. This developed technique was fully applicable to analyze the enhanced production
of fucoxanthin isomers by iodine-catalyzed stereomutation which composed of 9 isomer species including 9-cis. | en_US |
