Effect of Acupuncture on ST36 Acite Stitches to Accessible Epitelization Accuracies in Rats of Rats Infected of MRSA Bacteria

Abstract

Epithelialization is part of skin tissue regeneration to repair the damage. This process will be inhibited by the presence of bacterial infections, especially Methicillin resistant Staphylococcus aureus (MRSA). This study aims to determine the effect of stabbing acupuncture needles on ST36 acupoint on epithelization on the healing process of MRSA infected wounds in the skin of wisting rats. Thirty six Wistar rats, 3 months old, are divided into 6 groups, 2 groups of negative control , 2 groups of positive control , 2 groups of treatment group (All are sacrificed on the fourth day and sixth day ) . Injury to the back skin of rats with a knife along the incised 2 cm and depth to subcutaneous. Wound of positive control groups infected by MRSA, wound in the treatment groups also infected MRSA and stabbing at ST36 acupoints. Each group are sacrificed to/for day 4 and 6, the skin tissue is fixed, made histological preparations, stained with HE. The measurement of the epithelial length using Optilab mounted on a light microscope. The data are analyzed by comparing the mean and SD. The epithelial length of the treatment group is higher than the positive control {(0,46±0,19 ) vs (0,21±0,16 );(0,63±0,76 ) vs (0,42±0,301 )}, being compared with the negative control is not much different. Stabbing acupuncture needles on ST36 acupoint accelerates epithelialization in wound healing of the skin MRSA-infected rat.