| dc.description.abstract | Sperm centrifugation is an important process to obtain good spermatozoa for assisted
reproductive techniques. However, the centrifugation process has additional impacts such as increased
reactive oxygen species (ROS) and oxidative stress affecting sperm viability due to damage of
spermatozoa membrane. The use of vitamin C (ascorbic acid) can reduce elevated ROS levels, thereby
protecting DNA and spermatozoa from damage caused by oxidative stress, meanwhile albumin can
reduce oxidative stress and maintain the integrity of spermatozoa membrane. This study aimed to
obtain the effect of combination of vitamin C and albumin on spermatozoa viability in centrifugation
process. This was a true experimental in vitro study using post-test-only control design. There were
four groups in this study, as follows: the control group (K); the treatment group (P1), centrifugation
without vitamin C and albumin; (P2), centrifugation with vitamin C; and (P3), centrifugation with a
combination of vitamin C and albumin. The Mann-Whitney test for each group showed significantly
differences in spermatozoa viability (p<0.05) among them. The treatment group receiving
supplementation with a combination vitamin C and albumin produced the highest viability after the
control group. In conclusion, supplementation of combination of vitamin C and albumin is capable in
maintaining spermatozoa in centrifugation process. | en_US |
