Efek Pemberian Suplementasi Kombinasi Vitamin C dan Albumin Terhadap Viabilitas Spermatozoa dalam Proses Sentrifugasi (Studi In Vitro)

Abstract

Sperm centrifugation is an important process to obtain good spermatozoa for assisted reproductive techniques. However, the centrifugation process has additional impacts such as increased reactive oxygen species (ROS) and oxidative stress affecting sperm viability due to damage of spermatozoa membrane. The use of vitamin C (ascorbic acid) can reduce elevated ROS levels, thereby protecting DNA and spermatozoa from damage caused by oxidative stress, meanwhile albumin can reduce oxidative stress and maintain the integrity of spermatozoa membrane. This study aimed to obtain the effect of combination of vitamin C and albumin on spermatozoa viability in centrifugation process. This was a true experimental in vitro study using post-test-only control design. There were four groups in this study, as follows: the control group (K); the treatment group (P1), centrifugation without vitamin C and albumin; (P2), centrifugation with vitamin C; and (P3), centrifugation with a combination of vitamin C and albumin. The Mann-Whitney test for each group showed significantly differences in spermatozoa viability (p<0.05) among them. The treatment group receiving supplementation with a combination vitamin C and albumin produced the highest viability after the control group. In conclusion, supplementation of combination of vitamin C and albumin is capable in maintaining spermatozoa in centrifugation process.